Micro-Flask - System Duetz

The System Duetz is an integrated and user-friendly technology known for its exceptional cost-effectiveness due to minimal volume usage and quick, easy startup for cultivations.

It allows for the cultivation of a large number of microbial strains, clone libraries, cell banks, or cell lines in 24 or 96 well plates simultaneously.

• The system enables one person to cultivate thousands of strains simultaneously with minimal repetitive tasks
•  Everything happens in parallel across 24 or 96 rows
•  Many users have reported reducing their time spent by more than a factor of 10
• The growth in the plates is of comparable quality to that in Erlenmeyer flasks in terms of oxygen transfer rate (OTR) and reproducibility
•  All of these claims are documented with technical data, images, and videos showcasing the hydrodynamics within the wells during orbital shaking

Sandwich lids are now available for:

• 24 square deepwell plates (2.5 - 4 ml vol.)
• 96 square deepwell plates (0.5 - 1 ml vol.)
• 24 round lowwell plates (0.75-1.5 ml vol.)
• 96 round lowwell plates (0.1 - 0.2 ml vol.)

Applications include:

• Research: High-throughput screening for new enzyme activity or secondary metabolite formation
• Library screening and distribution in E. coli or yeast
• High-throughput screening for highly active or productive mutants
• Comparative studies
• Studies in optimizing growth media.

Key components of the system:
Sandwich lids: Convert all wells in the microplate into individual mini-reactors, ideal for high-frequency orbital shaking with high oxygen transfer levels and minimal well-to-well variation.

Clamps: Ensure tight sealing between plates and sandwich lids, preventing cross-contamination.

The plates are placed in a shaking incubator with temperature, CO2, and humidity control.

For more information on the Micro-Flask - System Duetz, please visit Applikon´s website